NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(2) [April 2009] >


Title: A simplified method for extraction of high quality genomic DNA from Jatropha curcas for genetic diversity and molecular marker studies
Authors: Pamidimarri, D V N Sudheer
Meenakshi
Sarkar, Ritam
Boricha, Girish
Reddy, Muppala P
Keywords: CTAB
Jatropha curcas L
molecular markers
RAPD
AFLP
Issue Date: Apr-2009
Publisher: CSIR
Abstract:  A simple and efficient protocol for the extraction of high quality genomic DNA from different tissues, including callus generated from leaves of Jatropha curcas has been developed. The important steps in this protocol include (a) use of 3.5 M NaCl in extraction buffer; (b) 2.0 M NaCl (final concentration) during precipitation; (c) Tris saturated phenol in place of phenol:chloroform:isoamyl alcohol at purification phase; (d) 80% ethanol for DNA precipitation, and (e) performing all the steps at RT. The DNA thus extracted from the leaves had 1.81±0.063, OD at A260/280 and the yield was 120 to 140 µg/g of material. The extracted DNA was found suitable for restriction digestion, ligation and PCR amplification. It was also used for DNA fingerprinting techniques, RAPD and AFLP, for development of molecular markers and studies on genetic diversity.
Page(s): 187-192
ISSN: 0972-5849
Source:IJBT Vol.08(2) [April 2009]

Files in This Item:

File Description SizeFormat
IJBT 8(2) 187-192.pdf1.3 MBAdobe PDFView/Open
 Current Page Visits: 781 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 602983 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in