Rapid clonal multiplication through <i style="">in vitro </i>axillary shoot proliferation of <i style="">Centella asiatica </i>L.

Abstract

<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter"> Single nodal explants isolated from field grown plants of <i style="">Centella asiatica</i>, an important medicinal plant, when cultured for 4 wks on Murashige and Skoog’s (MS) medium containing different concentrations and combinations of BAP and Kn produced multiple shoots. A maximum of 15.24 shoots/node were produced after 30 d of culture in the presence of 2.0 mg/L BAP. Individual shoots (2-5 cm), when transferred onto full strength MS medium containing 1.5 mg/L IBA induced maximum number of roots (12.6). The rooted plants were successfully established in greenhouse condition after hardening. </smarttagtype>