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IJBT Vol.08(2) [April 2009] >


Title: Rapid clonal multiplication through in vitro axillary shoot proliferation of Centella asiatica L.
Authors: Karthikeyan, K
Chandran, C
Kulothungan, S
Keywords: Centella asiatica
medicinal plant
multiple shoot
in vitro
clonal multiplication
Issue Date: Apr-2009
Publisher: CSIR
Abstract:  Single nodal explants isolated from field grown plants of Centella asiatica, an important medicinal plant, when cultured for 4 wks on Murashige and Skoog’s (MS) medium containing different concentrations and combinations of BAP and Kn produced multiple shoots. A maximum of 15.24 shoots/node were produced after 30 d of culture in the presence of 2.0 mg/L BAP. Individual shoots (2-5 cm), when transferred onto full strength MS medium containing 1.5 mg/L IBA induced maximum number of roots (12.6). The rooted plants were successfully established in greenhouse condition after hardening.
Page(s): 232-235
ISSN: 0972-5849
Source:IJBT Vol.08(2) [April 2009]

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