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Title: Isolation and identification of poly beta hydroxybutyric acid accumulating bacteria of Staphylococcal sp. and characterization of biodegradable polyester
Authors: Roy, Bappaditya
Banerjee, Rajat
Chatterjee, Sumana
Keywords: Activated sludge;MALDI;NMR;Polyhydroxybutyric acid;Staphylococcus sp.;Thermoplastic biopolymer
Issue Date: Apr-2009
Publisher: CSIR
Abstract: Staphylococcus sp. strain BP/SU1, capable of degrading the biopolymer and utilize it as a source of carbon and energy, was isolated from activated sludge using METABOLIX (MBX D411G). It was found that this strain was capable of accumulating poly(3-hydroxybutyric acid) P(3-HB), as granule poly (3-hydroxybutyric acid), p(3-HB), inclusion bodies when grown under suitable nutrient conditions. These strains could sustain cell growth up to a dry mass of 9.24 g/l with a doubling time of 8 to 10 hr and could accumulate P(3-HB) as granular inclusion bodies to a cell dry weight of more than 12%. P(3-HB) accumulated by this organism was isolated and characterized through NMR, FT- IR spectroscopy, UV Spectroscopy, Mass spectroscopy and Differential Scanning Calorimetry. P(3-HB) granules so isolated showed physical and chemical properties that should be possessed by a superior quality thermoplastic biopolymer.
Page(s): 250-256
ISSN: 0019-5189
Appears in Collections:IJEB Vol.47(04) [April 2009]

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