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Title: Activation of tobacco leaf polyphenol oxidase by sodium dodecyl sulfate
Authors: Jiang, Hui
Shi, Chunhua
Xie, Yongshu
Xu, Xiaolong
Liu, Qingliang
Keywords: Sodium dodecyl sulfate (SDS);polyphenol oxidase;tobacco leaf;pH effect;temperature effect
Issue Date: Oct-2003
Publisher: CSIR
Abstract: The effect of sodium dodecyl sulfate (SDS) on purified tobacco leaf PPO (PPO II) was investigated at various pHs and temperatures. SDS increased the activity of PPO II due to the formation of SDS-PPO II complex, leading to conformational changes, thus making access to active center easier. The relationship between the activity and the molar ratio of SDS-PPO II to PPO II showed that the critical point reached a plateau of activity at the molar ratio of about 1.2. The pH had a significant effect on interaction between SDS and PPO II, as compared to PPO II. The optimum catalytic temperature of the complex rose by 10°C, suggesting that stabilization of the structure had been improved by the formation of complex.
Page(s): 350-353
ISSN: 0301-1208
Appears in Collections:IJBB Vol.40(5) [October 2003]

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