NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.40 [2003] >
IJBB Vol.40(5) [October 2003] >

Title: Activation of tobacco leaf polyphenol oxidase by sodium dodecyl sulfate
Authors: Jiang, Hui
Shi, Chunhua
Xie, Yongshu
Xu, Xiaolong
Liu, Qingliang
Keywords: Sodium dodecyl sulfate (SDS)
polyphenol oxidase
tobacco leaf
pH effect
temperature effect
Issue Date: Oct-2003
Publisher: CSIR
Abstract: The effect of sodium dodecyl sulfate (SDS) on purified tobacco leaf PPO (PPO II) was investigated at various pHs and temperatures. SDS increased the activity of PPO II due to the formation of SDS-PPO II complex, leading to conformational changes, thus making access to active center easier. The relationship between the activity and the molar ratio of SDS-PPO II to PPO II showed that the critical point reached a plateau of activity at the molar ratio of about 1.2. The pH had a significant effect on interaction between SDS and PPO II, as compared to PPO II. The optimum catalytic temperature of the complex rose by 10°C, suggesting that stabilization of the structure had been improved by the formation of complex.
Page(s): 350-353
ISSN: 0301-1208
Source:IJBB Vol.40(5) [October 2003]

Files in This Item:

File Description SizeFormat
IBB 40(5) 350-353.pdf83.51 kBAdobe PDFView/Open
 Current Page Visits: 80 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167098 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: