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|Title:||Changes in the activity of matrix metalloproteinases in regenerating rat liver after CCl<sub>4 </sub>-induced injury|
Sudhakaran, P R
matrix metalloproteinases (MMPs)
extracelluar matrix (ECM)
|Abstract:||Molecular mechanisms involved in mediating alteration in cell matrix interaction have been examined by studying the changes in the activity of matrix metalloproteinases (MMPs) in CCl<sub>4 </sub>-induced regenerating liver, using zymography and ELISA. Activity of MMPs (72 kD, 92 kD and 130 kD gelatinases) in the rat liver increased progressively during acute injury till the 4<sup>th</sup> day and then decreased to near normal level after CCl<sub>4 </sub> administration (0.5 ml/100 g body wt.) on the 6<sup>th</sup> day. Hepatocyte lysate of injured liver on the 4<sup>th</sup> day showed significantly higher levels of MMP2 and MMP9 compared to the control. In the culture medium of hepatocytes, the levels of MMP2 and MMP9 increased progressively with the duration of culture, indicating that hepatocytes are the major source of these MMPs in regenerating liver. These results suggest an involvement of MMPs in matrix degradation and remodeling during regeneration after acute liver injury induced by CCl<sub>4 </sub>.|
|Appears in Collections:||IJBB Vol.40(5) [October 2003]|
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