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IJBB Vol.40(4) [August 2003] >


Title: Purification and partial characterization of cytochrome c552 from Halobacterium salinarium
Authors: Sreeramulu, K
Keywords: Cytochrome c552
Halobacterium salinarium
purification
characterization
Issue Date: Aug-2003
Publisher: CSIR
Abstract: Cytochrome c552 was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be 14.1 kD.
Page(s): 274-277
ISSN: 0301-1208
Source:IJBB Vol.40(4) [August 2003]

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