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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.40 [2003] >
IJBB Vol.40(4) [August 2003] >
| Title: | Purification and partial characterization of cytochrome c552 from Halobacterium salinarium |
| Authors: | Sreeramulu, K |
| Keywords: | Cytochrome c552
Halobacterium salinarium
purification
characterization |
| Issue Date: | Aug-2003 |
| Publisher: | CSIR |
| Abstract: | Cytochrome c552 was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be
14.1 kD. |
| Page(s): | 274-277 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.40(4) [August 2003]
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