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Title: Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC)
Authors: Abadi, Reza Haji Hosseini Baghdad
Issue Date: Jun-2003
Publisher: CSIR
Abstract: In this paper, analysis of free nucleotides from mouse liver tis-sue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO-4, subjected to preliminary purification on a Cu2+-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure used in the study gave a good resolu-tion of free nucleotides on a single column.
Page(s): 209-212
ISSN: 0301-1208
Source:IJBB Vol.40(3) [June 2003]

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