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|Title:||Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC)|
|Authors:||Abadi, Reza Haji Hosseini Baghdad|
|Abstract:||In this paper, analysis of free nucleotides from mouse liver tis-sue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO<sup>-</sup><sub>4</sub>, subjected to preliminary purification on a Cu<sup>2+</sup>-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure used in the study gave a good resolu-tion of free nucleotides on a single column.|
|Appears in Collections:||IJBB Vol.40(3) [June 2003]|
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