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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.40 [2003] >
IJBB Vol.40(3) [June 2003] >
| Title: | Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC) |
| Authors: | Abadi, Reza Haji Hosseini Baghdad |
| Issue Date: | Jun-2003 |
| Publisher: | CSIR |
| Abstract: | In this paper, analysis of free nucleotides from mouse liver tis-sue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO-4, subjected to preliminary purification on a Cu2+-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure used in the study gave a good resolu-tion of free nucleotides on a single column. |
| Page(s): | 209-212 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.40(3) [June 2003]
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