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IJBB Vol.40(3) [June 2003] >


Title: Characterization of goat plasma vitronectin
Authors: Suchitra, S
Ashok, Vasili
Gupta, Tapas
Joshi, Paritosh
Keywords: Goat
vitronectin
Issue Date: Jun-2003
Publisher: CSIR
Abstract: Vitronectin (VN) was isolated and characterized from goat plasma in native and denatured state. Native VN consisted of 160 and >250 kDa polypeptides, whereas denatured VN showed bands of 81 and >250 kDa on SDS-gel. Storage of 81 kDa polypeptide for 3 days at 4ÂșC resulted in formation of 160 and >250 kDa proteins. Hence high molecular weight forms of VN may be dimer and multimeric forms of 81 kDa monomer. Both native as well as denatured VN showed cell adhesive activity. Cells bound to native VN were round, whereas cells adhered to denatured VN were fully spread, a characteristic also observed with 81 kDa polypeptide. The 81 kDa VN bound to Heparin, whereas the 160 kDa preparation did not bind to Heparin in presence of urea. Absence of EDTA resulted in the degradation of goat VN. Similarly, addition of excess Ca2+ caused total degradation of VN polypeptides in buffers with EDTA, suggesting metalloprotease activity in the protein.
Page(s): 186-193
ISSN: 0301-1208
Source:IJBB Vol.40(3) [June 2003]

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