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|Title:||Effects of deuterium oxide on folate metabolism in Lactobacillus casei|
|Authors:||Pote, M S|
|Abstract:||Various folate coenzymes and their polyglutamyl derivatives involved in 1-carbon metabolism are modulated as a result of altered physiological states and also vary with respect to growth conditions. We studied the metabolic changes in folic acid and their conjugated polyglutamyl derivatives in Lactobacillus casei cells grown in the presence of D<sub>2O</sub>. A 40% decrease in methyltetrahydrofolyl polyglutamate derivatives was observed in the cells grown in media prepared with D<sub>2O</sub>-depleted water (D<sub>2O</sub> content, 8-10 ppm). Chromatographic analysis of folates showed significant alterations in the formyl- and methyltetrahydrofolate derivatives and their polyglutamylation profiles. Higher amounts of oxidized folates were also present in the cells grown in D<sub>2O</sub>-depleted conditions. No significant changes were observed in folates and their polyglutamate derivatives when the cells were grown in the presence of 300, 450 and 600 ppm D<sub>2O</sub>. The altered folate homoestasis is attributed to changes in the metabolic adaptation of cells to D<sub>2O</sub>-depleted environment.|
|Appears in Collections:||IJBB Vol.40(3) [June 2003]|
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