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Title: Fluorescence spectroscopy in molecular description of biological processes
Authors: Krishnamoorthy, G
Keywords: 2-Aminopurine;cell membrane raft;DNA dynamics;Time-resolved fluorescence;fluorescence spectroscopy;fluorescence probes;Maximum entropy method;membrane heterogeneity;protein dynamics;protein folding
Issue Date: Jun-2003
Publisher: CSIR
Abstract: The power of fluorescence spectroscopy in enhancing our ability to obtain molecular description of complex biological processes is discussed. A brief account of sensitivity, selectivity and observable parameters of the various fluorescence methods and some typical applications which are mostly based on work in our laboratory are provided. The applications cover a wide range of areas such as protein dynamics, protein folding dynamics, protein-DNA interactions, membrane dy-namics and intracellular dynamics. Interesting information revealed by fluorescence-based methods in each of these applica-tions is highlighted.
Page(s): 147-159
ISSN: 0301-1208
Appears in Collections:IJBB Vol.40(3) [June 2003]

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