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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.40 [2003] >
IJBB Vol.40(2) [April 2003] >
| Title: | Identification of serotonin-sensitive aryl acylamidase activity with cobra venom acetylcholinesterase |
| Authors: | Rajesh, Ramanna V
Balasubramanian, Aiylam S
Boopathy, Rathanam |
| Issue Date: | Apr-2003 |
| Publisher: | CSIR |
| Abstract: | Acetylcholinesterase purified from cobra (Naja naja) venom exhibits a serotonin-sensitive aryl acylamidase activity. Both acetylcholinesterase and aryl acylamidase activities co-eluted in column chromatographic procedures (Sephadex G-75 and Zinc-Sepharose), co-migrated on polyacrylamide gel electrophoresis, co-immunoprecipitated by anti-snake venom anti-body and showed the same heat denaturation profile at 40°C. Further, several potent acetylcholinesterase inhibitors at differ-ent concentrations inhibited the cholinesterase and aryl acylamidase activities to the same extent. It is concluded that in co-bra venom, acetylcholinesterase is associated with a serotonin-sensitive aryl acylamidase activity similar to earlier observa-tions made with acetylcholinesterase from different sources. |
| Page(s): | 92-97 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.40(2) [April 2003]
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