Please use this identifier to cite or link to this item:
|Title:||Identification of serotonin-sensitive aryl acylamidase activity with cobra venom acetylcholinesterase|
|Authors:||Rajesh, Ramanna V|
Balasubramanian, Aiylam S
|Abstract:||Acetylcholinesterase purified from cobra (Naja naja) venom exhibits a serotonin-sensitive aryl acylamidase activity. Both acetylcholinesterase and aryl acylamidase activities co-eluted in column chromatographic procedures (Sephadex G-75 and Zinc-Sepharose), co-migrated on polyacrylamide gel electrophoresis, co-immunoprecipitated by anti-snake venom anti-body and showed the same heat denaturation profile at 40°C. Further, several potent acetylcholinesterase inhibitors at differ-ent concentrations inhibited the cholinesterase and aryl acylamidase activities to the same extent. It is concluded that in co-bra venom, acetylcholinesterase is associated with a serotonin-sensitive aryl acylamidase activity similar to earlier observa-tions made with acetylcholinesterase from different sources.|
|Appears in Collections:||IJBB Vol.40(2) [April 2003]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.