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Title: Purification and properties of glucose 6-phosphate dehydrogenase from turkey erythrocytes
Authors: Yılmaz, Hayrullah
Beydemir, Sukru
Bakan, Ebubekir
Kufrevioglu, O Irfan
Issue Date: Feb-2003
Publisher: CSIR
Abstract: Glucose 6-phosphate dehydrogenase (G6PD) was purified from turkey erythrocytes by ammonium sulphate precipitation and followed by ADP Sepharose affinity gel chromatography. The yield was 49.71% and specific activity of the enzyme was found to be 44.16 EU/mg protein. By gel filtration the molecular mass was found to be 75 kDa. The enzyme had an optimum pH at 9.0, and optimum temperature at 50oC. Km and Vmax for NADP+ and glucose 6- phosphate (G6-P) as substrates were also determined and effects of inhibitors such as ATP, NADH and NADPH were examined.
Page(s): 62-65
ISSN: 0301-1208
Appears in Collections:IJBB Vol.40(1) [February 2003]

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