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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.40 [2003] >
IJBB Vol.40(1) [February 2003] >
| Title: | Changes in NMR relaxation times in soybean and wheat seeds equilibrated at different temperatures and relative humidity |
| Authors: | Krishnan, P Nagarajan, S Moharir, A V |
| Issue Date: | Feb-2003 |
| Publisher: | CSIR |
| Abstract: | The relationship between equilibration injury and equilibration dependence of the transverse relaxation time (T2) meas-urements was examined using NMR in two different seed species (sensitive-soybean and tolerant-wheat) differing in their sensitivity to seed equilibration conditions. The T2 values of both seed species declined with high temperature (45oC) and low RH (5.5-1%) and, also with high temperature (45oC) and high RH (74.5-100%) conditions. A comparison of injury based on electrolyte leakage, seed germination percentage and T2 indicated that membrane permeability increased both at high temperature (45oC) and low RH (5.5-1%) and high temperature (45oC) and high RH (74.5-100%) seed equilibration conditions. There was an increase in T2 until 11.5% and 5.5% RH in soybean and wheat species respectively, followed by a decline. Loss of seed viability during equilibration at very low RH (5.5-1%) at 45oC, and similarly at high RH (74.5-100%) at 45oC indicates that the changes in T2 are probably due to the loss of membrane injury. |
| Page(s): | 46-50 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.40(1) [February 2003]
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