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Title: Changes in NMR relaxation times in soybean and wheat seeds equilibrated at different temperatures and relative humidity
Authors: Krishnan, P
Nagarajan, S
Moharir, A V
Issue Date: Feb-2003
Publisher: CSIR
Abstract: The relationship between equilibration injury and equilibration dependence of the transverse relaxation time (T<sub>2</sub>) meas-urements was examined using NMR in two different seed species (sensitive-soybean and tolerant-wheat) differing in their sensitivity to seed equilibration conditions. The T<sub>2</sub> values of both seed species declined with high temperature (45<sup>o</sup>C) and low RH (5.5-1%) and, also with high temperature (45<sup>o</sup>C) and high RH (74.5-100%) conditions. A comparison of injury based on electrolyte leakage, seed germination percentage and T<sub>2</sub> indicated that membrane permeability increased both at high temperature (45<sup>o</sup>C) and low RH (5.5-1%) and high temperature (45<sup>o</sup>C) and high RH (74.5-100%) seed equilibration conditions. There was an increase in T<sub>2</sub> until 11.5% and 5.5% RH in soybean and wheat species respectively, followed by a decline. Loss of seed viability during equilibration at very low RH (5.5-1%) at 45<sup>o</sup>C, and similarly at high RH (74.5-100%) at 45<sup>o</sup>C indicates that the changes in T<sub>2</sub> are probably due to the loss of membrane injury.
Description: 46-50
ISSN: 0301-1208
Appears in Collections:IJBB Vol.40(1) [February 2003]

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