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Title: Crystal structure of human seminal diferric lactoferrin at 3.4 Å resolution
Authors: Kumar, Janesh
Weber, Wolfgang
Münchau, Sabine
Yadav, Savita
Singh, S Bhaskar
Saravanan, K
Paramasivam, M
Sharma, Sujata
Kaur, Punit
Bhushan, A
Srinivasan, A
Betzel, Christian
Singh, T P
Issue Date: Feb-2003
Publisher: CSIR
Abstract: Lactoferrin was purified from human seminal fluid obtained from the semen bank. The purified samples were saturated with Fe3+ and crystallized by microdialysis method. The crystals belong to orthorhombic space group P212121 with a = 55.9 Å, b = 97.2 Å, c = 156.1 Å and Z = 4. The structure was determined with molecular replacement method and refined to an R factor of 18.7% for all the data to 3.4 Å resolution. The overall structure of seminal lactoferrin is similar to human colostrum lactoferrin. The amino acid sequence of seminal lactoferrin shows that it has one amino acid less than human colostrum lactoferrin and the structure of its N-terminal region is far more ordered than other lactoferrins. The structure of the iron-binding site and its immediate surroundings indicate well defined features.
Page(s): 14-21
ISSN: 0301-1208
Source:IJBB Vol.40(1) [February 2003]

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