Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/3730
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dc.contributor.authorKalia, Vandana-
dc.contributor.authorPundir, Chandra S-
dc.date.accessioned2009-04-02T10:58:59Z-
dc.date.available2009-04-02T10:58:59Z-
dc.date.issued2004-12-
dc.identifier.issn0301-1208-
dc.identifier.urihttp://hdl.handle.net/123456789/3730-
dc.description326-328en_US
dc.description.abstractA method for determination of serum triglycerides (Tgs) using lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase co-immobilized onto alkylamine glass beads (pore diameter 55 nm) through glutaralde¬hyde coupling was developed and evaluated. The minimum detection limit of the method was 0.54 mM. The analytical recovery of added triolein in the serum was 97.55±1.5% (mean ± S.D.). The mean value of serum Tgs, determined by the present method showed a good correlation (r=0.984) with the Bayer’s kit method, employing free enzymes. The within and between batch coefficients of variation (CV) were <2.25% and <1.35% respectively. No significant loss of activity was observed, when co-immobilized enzymes were reused for about 200 times and stored at 4°C in distilled water. The cost of Tg determination for 200 serum samples was less, as compared with Bayer’s kit method.en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.relation.ispartofseriesC12 Q 1en_US
dc.relation.ispartofseries28en_US
dc.relation.ispartofseriesC12Q 1en_US
dc.relation.ispartofseries61en_US
dc.sourceIJBB Vol.41(6) [December 2004]en_US
dc.subjectTriglycerideen_US
dc.subjectlipaseen_US
dc.subjectglycerol kinaseen_US
dc.subjectglycerol-3-phosphate oxidaseen_US
dc.subjectco-immobilizationen_US
dc.subjectalkylamine glasen_US
dc.subjectserumen_US
dc.titleDetermination of serum triglycerides using lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase co-immobilized onto alkylamine glass beadsen_US
dc.typeArticleen_US
Appears in Collections:IJBB Vol.41(6) [December 2004]

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