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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(6) [December 2004] >
| Title: | Determination of serum triglycerides using lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase co-immobilized onto alkylamine glass beads |
| Authors: | Kalia, Vandana
Pundir, Chandra S |
| Keywords: | Triglyceride
lipase
glycerol kinase
glycerol-3-phosphate oxidase
co-immobilization
alkylamine glas
serum |
| Issue Date: | Dec-2004 |
| Publisher: | CSIR |
| IPC Code: | C12 Q 1
28
C12Q 1
61 |
| Abstract: | A method for determination of serum triglycerides (Tgs) using lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase co-immobilized onto alkylamine glass beads (pore diameter 55 nm) through glutaralde¬hyde coupling was developed and evaluated. The minimum detection limit of the method was 0.54 mM. The analytical recovery of added triolein in the serum was 97.55±1.5% (mean ± S.D.). The mean value of serum Tgs, determined by the present method showed a good correlation (r=0.984) with the Bayer’s kit method, employing free enzymes. The within and between batch coefficients of variation (CV) were <2.25% and <1.35% respectively. No significant loss of activity was observed, when co-immobilized enzymes were reused for about 200 times and stored at 4°C in distilled water. The cost of Tg determination for 200 serum samples was less, as compared with Bayer’s kit method. |
| Page(s): | 326-328 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.41(6) [December 2004]
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