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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(6) [December 2004] >
| Title: | Automated derivatization with o-phthalaldehyde for the estimation of amino acids in plasma using reversed-phase high performance liquid chromatography |
| Authors: | Gnanou, J V
Srinivas, S K
Kurpad, A V |
| Keywords: | o-Phthaladehyde derivatization
reversed-phase high performance liquid chromatography
plasma amino acid analysis. |
| Issue Date: | Dec-2004 |
| Publisher: | CSIR |
| IPC Code: | G01 N 33
88 |
| Abstract: | A fully automated method for quantitative estimation of plasma amino acids using fluorescence detection of o-phthaladehyde/2-mercaptoethanol derivatives of the analytes and their separation by gradient elution reversed-phase HPLC has been described. The method is simple and the three-step gradient elution is suitable for routine analysis of a large number of biological samples due to clear resolution, high degree of precision, accuracy, cost-effectiveness and lack of interference from chemical contaminations. Using this method, 19 amino acids were completely resolved and the within-run coefficients of variation ranged from 2.53 to 10.7% with a mean variation of 5.68%. |
| Page(s): | 322-325 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.41(6) [December 2004]
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