NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(6) [December 2004] >

Title: Automated derivatization with o-phthalaldehyde for the estimation of amino acids in plasma using reversed-phase high performance liquid chromatography
Authors: Gnanou, J V
Srinivas, S K
Kurpad, A V
Keywords: o-Phthaladehyde derivatization
reversed-phase high performance liquid chromatography
plasma amino acid analysis.
Issue Date: Dec-2004
Publisher: CSIR
IPC CodeG01 N 33
Abstract: A fully automated method for quantitative estimation of plasma amino acids using fluorescence detection of o-phthaladehyde/2-mercaptoethanol derivatives of the analytes and their separation by gradient elution reversed-phase HPLC has been described. The method is simple and the three-step gradient elution is suitable for routine analysis of a large number of biological samples due to clear resolution, high degree of precision, accuracy, cost-effectiveness and lack of interference from chemical contaminations. Using this method, 19 amino acids were completely resolved and the within-run coefficients of variation ranged from 2.53 to 10.7% with a mean variation of 5.68%.
Page(s): 322-325
ISSN: 0301-1208
Source:IJBB Vol.41(6) [December 2004]

Files in This Item:

File Description SizeFormat
IJBB 41(6) 322-325.pdf61.55 kBAdobe PDFView/Open
 Current Page Visits: 76 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167101 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: