Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/3724
Title: cAMP-mediated upregulation of gelatinases in primary cultures of isolated rat hepatocytes
Authors: Saja, K
Sudhakaran, P R
Keywords: Rat hepatocytes
matrix metalloproteinases (MMPs)
tissue inhibitor of MMP (TIMP)
cAMP-mediated upregulation
extracellular matrix (ECM)
horse radish peroxidase
Issue Date: Dec-2004
Publisher: CSIR
Series/Report no.: C 12 Q 1
37
Abstract: Matrix metalloproteinases (MMPs) play a major role in tissue remodelling and repair in pathophysiological conditions, such as liver fibrosis and regeneration. Regulation of the MMPs produced by liver cells is important in maintaining cell-matrix ratio in liver, which is a major target site for hormones that mediate their intracellular effects through cAMP. The possibility of cAMP affecting the activity of MMPs and their endogenous inhibitors, tissue inhibitor of MMPs (TIMPs) was studied using isolated rat hepatocytes in culture. Zymographic analysis showed that treatment with hormones like epinephrine, thyroxine and dexamethasone and Bt<sub>2</sub> cAMP increased 92 kDa MMP-9 activity. Bt<sub>2</sub> cAMP caused upregulation of MMP-9 in a dose-dependent manner. The effect of hormones was less on MMP-2. ELISA using specific antibodies showed increase in levels of MMP-9 and TIMP-1 protein. Kinetic analysis of production of MMPs and TIMPs showed that the response to Bt<sub>2</sub> cAMP was a delayed one, indicating its effect on de novo protein synthesis. These results suggest the possibility of cAMP dependent regulation of MMP-9 in the hepatocytes.
Description: 294-298
URI: http://hdl.handle.net/123456789/3724
ISSN: 0301-1208
Appears in Collections:IJBB Vol.41(6) [December 2004]

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