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Title: Iron dependence and zinc inhibition of duodenal cytosolic aconitase of rat
Authors: Sreedhar, Bodiga
Nair, K Madhavan
Keywords: Fe deficiency
cytosolic aconitase
duodenum, zinc
Issue Date: Oct-2004
Publisher: CSIR
Abstract: The response of duodenal cytosolic aconitase (c-aconitase) to oral repletion of graded doses of iron (Fe) during Fe-deficiency was studied in rats (WNIN strain). In addition, in vitro effect of zinc (Zn) on the enzyme activity was studied using duodenal cytosol. Iron-depleted male rats were orally repleted with either 100 or 190 or 370 μg of Fe/day (n=6, each) for 2 weeks. Fe repletion was found to increase linearly the activity of duodenal c-aconitase along with the indicators of iron status. The correlation coefficient (r) between c-aconitase and haemoglobin and mucosal ferritin was 0.6453 and 0.8441, respectively. The effects of zinc (0-40 μM) in vitro on the kinetics of c-aconitase from iron-replete stock diet fed rats (n=4) showed that Zn competitively inhibited the enzyme with a Ki (app.) of 28 μM. These observations suggest that c-aconitase is a critical target involved in the assimilation of Fe and excess dietary Zn can result in negative interactions.
Page(s): 250-253
ISSN: 0301-1208
Source:IJBB Vol.41(5) [October 2004]

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