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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(5) [October 2004] >
| Title: | Iron dependence and zinc inhibition of duodenal cytosolic aconitase of rat |
| Authors: | Sreedhar, Bodiga
Nair, K Madhavan |
| Keywords: | Fe deficiency
cytosolic aconitase
duodenum, zinc
rat |
| Issue Date: | Oct-2004 |
| Publisher: | CSIR |
| Abstract: | The response of duodenal cytosolic aconitase (c-aconitase) to oral repletion of graded doses of iron (Fe) during Fe-deficiency was studied in rats (WNIN strain). In addition, in vitro effect of zinc (Zn) on the enzyme activity was studied using duodenal cytosol. Iron-depleted male rats were orally repleted with either 100 or 190 or 370 μg of Fe/day (n=6, each) for 2 weeks. Fe repletion was found to increase linearly the activity of duodenal c-aconitase along with the indicators of iron status. The correlation coefficient (r) between c-aconitase and haemoglobin and mucosal ferritin was 0.6453 and 0.8441, respectively. The effects of zinc (0-40 μM) in vitro on the kinetics of c-aconitase from iron-replete stock diet fed rats (n=4) showed that Zn competitively inhibited the enzyme with a Ki (app.) of 28 μM. These observations suggest that c-aconitase is a critical target involved in the assimilation of Fe and excess dietary Zn can result in negative interactions. |
| Page(s): | 250-253 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.41(5) [October 2004]
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