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Title: Kinetic studies on thermal denaturation of C-phycocyanin
Authors: Patel, Anamika
Pawar, Richa
Mishra, Sandhya
Sonawane, Shailendra
Ghosh, P K
Keywords: Phycobiliproteins
Spirulina platensis
thermal denaturation
first order kinetics
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeA 23 J 3
A 61 K 35
Abstract: The kinetics of thermal denaturation of a biliprotein, C-phycocyanin (C-PC) isolated from Spirulina platensis were studied at different pH values, ranging from 4.0 to 8.0. The denaturation of C-PC follows the first order kinetics and rate constant at pH 5.0 and temperature 55ºC is found to be 4.37 X 10-5 s-1, which increases to 5.46 X 10-5 s-1 at pH 7.0. The denaturation rate is much higher at 65ºC and pH 7.0 (7.96 X 10-4), as compared to at pH 5.0 (1.46 X 10-4). The thermal stability of C-PC is more at pH 5.0, as compared to other pH values. The observed differences in entropy values at pH 5.0, as compared to other pH values indicate a considerably close fit structure of the protein at pH 5.0, which increases the stability of native structure, even at higher temperature (65ºC).
Page(s): 254-257
ISSN: 0301-1208
Source:IJBB Vol.41(5) [October 2004]

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