NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(5) [October 2004] >


Title: Kinetic studies on thermal denaturation of C-phycocyanin
Authors: Patel, Anamika
Pawar, Richa
Mishra, Sandhya
Sonawane, Shailendra
Ghosh, P K
Keywords: Phycobiliproteins
Spirulina platensis
C-Phycocyanin
thermal denaturation
first order kinetics
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeA 23 J 3
20
A 61 K 35
80
Abstract: The kinetics of thermal denaturation of a biliprotein, C-phycocyanin (C-PC) isolated from Spirulina platensis were studied at different pH values, ranging from 4.0 to 8.0. The denaturation of C-PC follows the first order kinetics and rate constant at pH 5.0 and temperature 55ºC is found to be 4.37 X 10-5 s-1, which increases to 5.46 X 10-5 s-1 at pH 7.0. The denaturation rate is much higher at 65ºC and pH 7.0 (7.96 X 10-4), as compared to at pH 5.0 (1.46 X 10-4). The thermal stability of C-PC is more at pH 5.0, as compared to other pH values. The observed differences in entropy values at pH 5.0, as compared to other pH values indicate a considerably close fit structure of the protein at pH 5.0, which increases the stability of native structure, even at higher temperature (65ºC).
Page(s): 254-257
ISSN: 0301-1208
Source:IJBB Vol.41(5) [October 2004]

Files in This Item:

File Description SizeFormat
IJBB 41(5) 254-257.pdf150.6 kBAdobe PDFView/Open
 Current Page Visits: 525 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 513174 since 06-Feb-2009  Last updated on 21-Apr-2014Webmaster: nopr@niscair.res.in