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Title: Developmental changes in intestinal brush border enzymes of rats prenatally exposed to ethanol
Authors: Bhalla, Sonali
Kaur, Kamaljit
Mahmood, Safrun
Mahmood, Akhtar
Keywords: Prenatal
ethanol exposure
intestinal brush border enzymes
postnatal development
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeC12Q 1/37
C12Q 1/42
Abstract: The activities of lactase, sucrase, alkaline phosphatase (AP) and ϒ-glutamyl transpeptidase (ϒ-GTP) were studied in the intestinal brush border membranes of pups born to rat mothers exposed to ethanol (1 ml of 30% ethanol daily during gestation) at different days of postnatal development. The activities of lactase (at day 4-20) and sucrase (at day 20-30) were considerably reduced in response to prenatal exposure to ethanol, while AP (at day 4-30) and ϒ-GTP activities were significantly enhanced (p<0.05) at day 4, 8, 14 and 20, but there was no significant difference by day 30 of postnatal development. The observed changes in enzyme activities were corroborated by Western blot analysis of lactase, sucrase and AP. Kinetic studies revealed a change in Vmax without affecting apparent Km of enzymes under these conditions. The present findings suggest that in utero ethanol exposure to rats is embryotoxic and affects the postnatal development of various brush border enzymes, which persist long after the ethanol was withdrawn prior to birth.
Page(s): 221-226
ISSN: 0301-1208
Source:IJBB Vol.41(5) [October 2004]

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