NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(5) [October 2004] >


Title: Purification, characterization and antigenic species-specific reactivity of vitellogenin of rosy barb (Puntius conchonius Hamilton)
Authors: Shi, Xiaodong
Zhang, Shicui
Sun, Yaning
Pang, Qiuxiang
Sawant, M S
Keywords: Rosy barb
vitellogenin (Vg)
characterization
immunodiffusion
phospholipoglycoprotein
Puntius conchonius
purification
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeC 12 H 1/056
C 12 H1/469
Abstract: Vitellogenin (Vg) was isolated using gel filtration and ion-exchange chromatography from plasma of rosy barb (Puntius conchonius) treated with estrogen (estradiol-17β). The purified Vg was stained positive for carbohydrate, lipid and phosphorus and was rich in Ala (10.58%), Asp (8.46%), Glu (10.30%), Leu (11.23%), Lys (7.22%) and Val (7.49%). It appeared as a single band of approximately 450 kDa in native PAGE and was reduced to a single band of approximately 167 kDa under SDS-PAGE, suggesting that it is probably composed of three identical polypeptide subunits. Double-immunodiffusion assay showed that the plasma from female rosy barb reacted with the mouse antisera against rosy barb Vg, forming a single immunoprecipitin line, while the plasma from male rosy barb or female zebrafish showed no such reactivity, confirming the existence of the sex- and species-specific reactivity for rosy barb Vg antisera.
Page(s): 216-220
ISSN: 0301-1208
Source:IJBB Vol.41(5) [October 2004]

Files in This Item:

File Description SizeFormat
IJBB 41(5) 216-220.pdf413.27 kBAdobe PDFView/Open
 Current Page Visits: 511 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 576412 since 06-Feb-2009  Last updated on 26-Aug-2014Webmaster: nopr@niscair.res.in