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Title: Mechanism of regulation of phosphorylation-dephosphorylation of Ca²⁺, Mg²⁺- and Ca²⁺-ATPases by modulator proteins isolated from rat brain cytosol
Authors: Bhattacharyya, Dipankar
Ghosh, Subho
Chatterjee, Madhumouli
Basu, Arindam
Sen, Parimal C
Keywords: Phosphorylation
Ca²⁺, Mg²⁺- and Ca²⁺-ATPases
Modulator proteins
Rat brain cytosol
Issue Date: Aug-2004
Publisher: CSIR
Abstract: Two proteins of molecular mass 13 kDa, a specific inhibitor of Na⁺, K⁺-ATPase and another of 12 kDa, which can distinguish between Ca²⁺, Mg²⁺- and Ca²⁺-ATPase activities have been obtained from the pooled fractions isolated from rat brain, using Sephadex G-100 chromatography. In order to determine the key step(s), which is affected by the modulators, we have designed an in vitro experiment of phosphorylation and dephosphorylation of these ATPases in the absence and presence of the modulators. The results suggest that the phosphorylation step of Mg²⁺-independent Ca²⁺-ATPase is inhibited, while in Mg²⁺-dependent Ca²⁺-ATPase, the dephosphorylation step is stimulated by the modulators. The findings support our earlier observation that the modulators are able to distinguish between Mg²⁺-independent and dependent Ca²⁺-ATPases activities.
Page(s): 162-166
ISSN: 0301-1208
Source:IJBB Vol.41(4) [August 2004]

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