|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(4) [August 2004] >
| Title: | Antitumour activity of Annona squamosa seed extracts is through the generation of free radicals and induction of apoptosis |
| Authors: | Pardhasaradhi, B V V
Reddy, Madhurima
Ali, A Mubarak
Kumari, A Leela
Khar, Ashok |
| Keywords: | Annona squamosa
reactive oxygen species (ROS)
GSH
antitumour activity
apoptosis
Bcl-2
seed extract |
| Issue Date: | Aug-2004 |
| Publisher: | CSIR |
| Abstract: | The plant, Annona squamosa traditionally known as custard apple possesses potent bioactive principles in all its parts. The effect of aqueous and organic extracts from defatted seeds of A. squamosa was studied on a rat histiocytic tumour cell line, AK-5. Both the extracts caused significant apoptotic tumour cell death with enhanced caspase-3 activity, down regulation of antiapoptotic genes Bcl-2 and BclXL, and enhanced the generation of intracellular ROS, which correlated well with the decreased levels of intracellular GSH. In addition, DNA fragmentation and annexin-V staining confirmed that the extracts induced apoptosis in tumour cells through the oxidative stress. Aqueous extracts of A. squamosa seeds possessed significant antitumor activity in vivo against AK-5 tumor. |
| Page(s): | 167-172 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.41(4) [August 2004]
|
|
