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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.45 [2008] >
IJBB Vol.45(1) [February 2008] >
| Title: | Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation |
| Authors: | Sunil, B
Riazunnisa, K
Krishna, T Sai
Schansker, Gert
Strasser, Reto J
Raghavendra, Agepati S
Mohanty, Prasanna |
| Keywords: | Chl ⍺ fluorescence
Functional integrity
Mesophyll protoplasts
O-J-I-P Transients
Pisum sativum
Protoplast isolation |
| Issue Date: | Feb-2008 |
| Publisher: | CSIR |
| Abstract: | Intact and metabolically very active mesophyll protoplasts were isolated rapidly from pea (Pisum sativum) leaves. The functional performance of protoplasts at various stages of their isolation was analyzed by using fast Chl αfluorescence OJIP transients and compared with that of intact leaves. The results demonstrated that the OJIP transients could successfully be used to monitor the quality of mesophyll protoplasts at different isolation steps. The protoplasts maintained their integrity and photosynthetic status very well, and their performance was very similar to that of the intact leaves. |
| Page(s): | 37-43 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.45(1) [February 2008]
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