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Title: Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation
Authors: Sunil, B
Riazunnisa, K
Krishna, T Sai
Schansker, Gert
Strasser, Reto J
Raghavendra, Agepati S
Mohanty, Prasanna
Keywords: Chl ⍺ fluorescence
Functional integrity
Mesophyll protoplasts
O-J-I-P Transients
Pisum sativum
Protoplast isolation
Issue Date: Feb-2008
Publisher: CSIR
Abstract: Intact and metabolically very active mesophyll protoplasts were isolated rapidly from pea (Pisum sativum) leaves. The functional performance of protoplasts at various stages of their isolation was analyzed by using fast Chl αfluorescence OJIP transients and compared with that of intact leaves. The results demonstrated that the OJIP transients could successfully be used to monitor the quality of mesophyll protoplasts at different isolation steps. The protoplasts maintained their integrity and photosynthetic status very well, and their performance was very similar to that of the intact leaves.
Description: 37-43
ISSN: 0301-1208
Appears in Collections:IJBB Vol.45(1) [February 2008]

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