NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.45 [2008] >
IJBB Vol.45(1) [February 2008] >


Title: Cloning and characterization of diacylglycerol acyltransferase (DGAT) cDNA sequence from Brassica juncea cv. Pusa Bold
Authors: Ilaiyaraja, N
Rajarani, A P
Santha, I M
Keywords: Diacylglycerol acyltransferase (DGAT)
Kennedy pathway
Triacylglycerol
RT-PCR
cDNA cloning
Issue Date: Feb-2008
Publisher: CSIR
Abstract: Diacylgycerol acyltransferase (DGAT: EC 2.3.1.20) is the only enzyme in the Kennedy pathway that is exclusively committed to the synthesis of storage oil in plants. In this study, cloning and characterization of DGAT gene sequence from Brassica juncea cv Pusa bold, an important oil seed crop of India is reported. A partial gene sequence of 2003 bp was PCR amplified and cloned from B. juncea. Sequence analysis showed that it has 10 exonic and 9 intronic sequences in the partial gene. Two cDNA sequences namely BjDGAT 1 and BjDGAT 2 (1.5 kb) encoding DGAT enzymes were amplified by RT-PCR from the developing seeds. The complete length of these two cDNAs as determined by RACE technique was 1768 bp, including 5’ and 3’-UTR. Comparative analysis of the sequences showed that BjDGAT1 was 85.1% and 96% identical to BjDGAT2 across 1512 coding region and 503 overlapping deduced amino acids respectively. These proteins were alkaline in nature (pI, 8.5-8.6), having similar molecular size (56-57 kD), an N-terminal hydrophilic segment and 9 transmembrane segments. Diacylglycerol/phorbol ester-binding motif (HKWXXRHXYXP) and acyl CoA binding motif (FYXDWWN) required for binding of substrates remained conserved in these proteins. Expression of the two transcripts of DGAT and their role in oil biosynthesis can further be studied.
Page(s): 30-36
ISSN: 0301-1208
Source:IJBB Vol.45(1) [February 2008]

Files in This Item:

File Description SizeFormat
IJBB 45(1) (2008) 30-36.pdf382.23 kBAdobe PDFView/Open
 Current Page Visits: 906 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 621767 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in