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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.41 [2004] >
IJBB Vol.41(2&3) [April-June 2004] >
| Title: | Measurement of urinary oxalate by grain sorghum leaf oxalate oxidase immobilized to affixed alkylamine glass beads |
| Authors: | Kumari, M
Pundir, C S |
| Keywords: | Oxalate
Oxalate oxidase
Urine
Grain Sorghum
Alkylamine glass
Immobilized enzyme |
| Issue Date: | Apr-2004 |
| Publisher: | CSIR |
| Abstract: | Oxalate in urine was measured by grain Sorghum leaf oxalate oxidase conjugated to alkyl amine glass beads affixed in a beaker. The minimum detection limit was 0.05 mM/L in urine. Recovery of added oxalate in urine was 80.5% and within and between assay, coefficients of variation (CV) were <4% and <5.5%, respectively. Urinary oxalate values obtained by the present method showed a good correlation (r = 0.947) with those by Sigma kit method. The method is not only free from tedious handling of free glass beads and Cl⁻ interference, but also has longer stability and reusability of immobilized enzyme compared to that of barley root and forage Sorghum leaf. |
| Page(s): | 102-106 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.41(2&3) [April-June 2004]
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