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IJBB Vol.41(2&3) [April-June 2004] >


Title: Measurement of urinary oxalate by grain sorghum leaf oxalate oxidase immobilized to affixed alkylamine glass beads
Authors: Kumari, M
Pundir, C S
Keywords: Oxalate
Oxalate oxidase
Urine
Grain Sorghum
Alkylamine glass
Immobilized enzyme
Issue Date: Apr-2004
Publisher: CSIR
Abstract: Oxalate in urine was measured by grain Sorghum leaf oxalate oxidase conjugated to alkyl amine glass beads affixed in a beaker. The minimum detection limit was 0.05 mM/L in urine. Recovery of added oxalate in urine was 80.5% and within and between assay, coefficients of variation (CV) were <4% and <5.5%, respectively. Urinary oxalate values obtained by the present method showed a good correlation (r = 0.947) with those by Sigma kit method. The method is not only free from tedious handling of free glass beads and Cl⁻ interference, but also has longer stability and reusability of immobilized enzyme compared to that of barley root and forage Sorghum leaf.
Page(s): 102-106
ISSN: 0301-1208
Source:IJBB Vol.41(2&3) [April-June 2004]

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