Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/35830
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMishra, S S-
dc.date.accessioned2016-10-19T11:29:52Z-
dc.date.available2016-10-19T11:29:52Z-
dc.date.issued1998-06-
dc.identifier.issn0975-1033 (Online); 0379-5136 (Print)-
dc.identifier.urihttp://nopr.niscair.res.in/handle/123456789/35830-
dc.description222-226en_US
dc.description.abstractDot immunoasay (DIA) method was used to detect Vibrio alginolyticus and Aeromonas hydrophila in samples collected from infected shrimps Penaeus monodon and fishes Mugil cephalus. Bacterial cultures (OD650 = 0.5) were diluted to 1:40 and 5 µl were spotted onto nitrocellulose paper (NCP). The NCPs were allowed to react separately with antiserum raised against V. alginolyticus and A. hydrophila. Out of 52 isolates from shrimp and 20 from fish, 19 and 6 isolates showed positive color reaction, respectively from shrimp and fishes. Cross reaction of V. alginolyticus antiserum was noted with V. parahaemolyticus, V. harveyi and V. anguillarum but not with A. hydrophila or Pseudomonas sp. Antiserum raised against A. hydrophila did not react with any Vibrio sp. or Pseudomonas sp. The test was found suitable for rapid detection of pathogenic bacteria in shrimps and fishes.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJMS Vol.27(2) [June 1998]en_US
dc.titleUse of dot immunoassay for rapid detection of pathogenic bacteria Vibrio alginolyticus and Aeromonas hydrophila from shrimps and fishesen_US
dc.typeArticleen_US
Appears in Collections:IJMS Vol.27(2) [June 1998]

Files in This Item:
File Description SizeFormat 
IJMS 27(2) 222-226.pdf451 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.