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|Title:||Somaclonal variation studies in Orthosiphon stamineus (Benth.) using SDS-PAGE|
Malar, T Renisheya Joy Jeba
|Keywords:||Biochemical marker;In vitro plantlet;Plant breeding;SDS-PAGE;Somaclonal variation|
|Abstract:||The present investigation was intended to reveal somaclonal variation in Orthosiphon stamineus Benth. using SDS-PAGE as a tool. For electrophoretic studies, the proteins were isolated from mother plants, in vitro plantlets raised through nodal segments and plantlets regenerated from the leaf-derived calli of Orthosiphon stamineus, and separated using SDS-PAGE. Mother plants and nodal-segment derived plantlets were confirmed for their genetic uniformity by expressing similar banding pattern in the gel system. The proteins PP12 (0.08), PP22 (0.15), PP32 (0.25) and PP51 (0.41) showed their unique presence in plantlets regenerated from leaf derived calli and confirmed the occurrence of somoclonal variants. These protein profiles would be used as a biochemical marker for plant breeding or genetic improvement programme in future to identify mother plants and somaclonal variants of O. stamineus.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.15(2) [April 2016]|
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