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Title: Nuclear and mitochondrial DNA markers based identification of blunthorn lobster Palinustus waguensis Kubo, 1963 from South-west coast of India
Authors: Chakraborty, Rekha Devi
Maheswarudu, G
Purushothaman, P
Kuberan, G
Sebastian, Jomon
Radhakrishnan, E V
Thangaraja, R
Keywords: Blunthorn lobster(Palinustus waguensis);Deepsea;Genetic markers;Phylogenetics;Taxonomy
Issue Date: Apr-2016
Publisher: NISCAIR-CSIR, India
Abstract: The present study reports the occurrence of Palinustus waguensis Kubo, 1963 from South-west coast of India on the basis of morphological as well as molecular data. The DNA sequences of two mitochondrial genes, large subunit ribosomal DNA (16S rDNA) and cytochrome oxidase I (COI), and two nuclear-protein coding genes, viz., phosphoenolpyruvate carboxykinase (PEPCK) and sodium-potassium ATPase α-subunit (NaK), were submitted in the GenBank. Morphological examination of the specimen confirmed the identity of the species as P. waguensis. The pairwise genetic distance analysis, sequence identity and phylogenetic tree have shown that the specimen has similarity only with 16S rDNA sequence of P. waguensis of earlier reports. In the case of other genetic markers, it has shown some identity towards P. unicornutus and P. holthuisi, but with a high genetic difference. This study with sequence data of P. waguensis in GenBank will help to identify the specimen on molecular basis in future.
Page(s): 172-177
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.15(2) [April 2016]

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