Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/35551
Title: Evaluation of new ‘indigenous milk dot-ELISA’ as ‘Field Test’ vis-à-vis milk plate-ELISA for the detection of Mycobacterium avium sub species paratuberculosis infection in lactating domestic livestock
Authors: Singh, Shoor Vir
Stephen, Bjorn John
Singh, Manju
Gupta, Saurabh
Chaubey, Kundan Kumar
Sahzad
Jayaraman, Sujata
Aseri, Gajendra Kumar
Sohal, Jagdip Singh
Bhatia, Ashok Kumar
Pachoori, Anjali
Chauhan, Jitendra
Dhama, Kuldeep
Keywords: Paratuberculosis;Dot-ELISA;Plate-ELISA;Milk;Lactating goats and cattle
Issue Date: Apr-2016
Publisher: NISCAIR-CSIR, India
Abstract: Mycobacterium avium subspecies paratuberculosis (MAP), the cause of incurable Johne’s disease (JD), is endemic in both domestic livestock and human population of the country. It is the major cause of low per animal productivity and reduced productive life in domestic livestock, wherein buffaloes, goats and sheep go for early slaughter for harvesting meat and cows are left to roam on the roads of cities and towns. In the present study, milk dot-ELISA test was standardized in efforts to develop a ‘field test’ for the detection of MAP infection in lactating animals using milk samples. Results of newly developed ‘milk dot-ELISA’ were compared with well standardized ‘indigenous milk plate-ELISA kit’, with known sensitivity and specificity. Of the 276 milk samples screened, 43 (32.0%) and 51 (35.9%) were positive in plate-ELISA and dot-ELISA (True Positives), for bovine and caprine paratuberculosis, respectively. Sensitivity and specificity of dot-ELISA vis-à-vis plate-ELISA was 86.2 and 73.8%, respectively. Newly standardized dot-ELISA was found to be highly sensitive, cost effective, quick, repeatable and efficient ‘field test’ for the screening of milk samples of lactating cattle, buffaloes, sheep and goats against MAP infection.
Page(s): 166-171
URI: http://nopr.niscair.res.in/handle/123456789/35551
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.15(2) [April 2016]

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