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Title: Expression of Δ¹-pyrroline-5-carboxylate synthetase gene during drought in rice (Oryza sativa L.)
Authors: Choudhary, N L
Sairam, R K
Tyagi, A
Keywords: Rice
Oryza sativa L.
Osmotic stress
Pyrroline-5-carboxylate synthetase
P5CS expression
Relative water content
Issue Date: Dec-2005
Publisher: CSIR
IPC CodeC12Q1/00, C12N15/52
Abstract: The 45-days-old seedlings of drought resistant (N-22, CR143-2-2) and susceptible rice (Oryza sativa L.) genotypes (Panidhan, Pusa-169) were subjected to osmotic stress in PEG-6000 solution of -10 and -16 bar and the relative water content (RWC), proline content, and pyrroline-5-carboxylate synthetase (P5CS) activity and its P5CS expression were studied. A gradual decrease in RWC was observed in tolerant genotypes, whereas the decrease was drastic in susceptible ones. Proline content and P5CS activity increased both in susceptible and tolerant genotypes; the increase was higher in tolerant genotypes. Higher proline levels in tolerant genotypes were due to increased P5CS activity. The EcoRI, BamHI and XbaI restricted DNA of N-22 and Panidhan genotypes were hybridized with Arabidopsis P5CS sequence and a single band (approx 2.4 kb) was observed, however, P5CS expression was more in N-22, as compared to Panidhan.
Page(s): 366-370
ISSN: 0301-1208
Source:IJBB Vol.42(6) [December 2005]

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