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IJBB Vol.42(6) [December 2005] >


Title: Antibacterial activity of crude methanolic ex-tract and its fractions of aerial parts of Anthemis tinctoria
Authors: Akgul, Cahit
Saglikoglu, Gulsen
Keywords: Anthemis tinctoria
Antibacterial activity
Pseudo-monas aeruginosa
Staphylococcus aureus
MIC
MBC
Issue Date: Dec-2005
Publisher: CSIR
IPC CodeA61P31/04
Abstract: The antibacterial activity of the methanolic extract and its frac-tions of aerial parts of Anthemis tinctoria (Asteraceae) was inves-tigated against representative gram-positive Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 29212) and gram-negative strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The activity was con-centrated mainly in the dichloromethane (DCM) and hexane fractions of crude methanolic extract. The 5 mg of DCM extract per disk produced 15-16 mm of inhibition zone against S. aureus and P. aeruginosa, however, no activity was found against E. faecalis and E. coli. The hexane fraction showed activity against S. aureus, P. aeruginosa and E. faecalis. As DCM fraction showed the highest antibacterial activity in the disk diffusion assay, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of only this fraction was determined against S. aureus and P. aeruginosa. These values were found to be in the range of 1.25 to 10 mg/ml.
Page(s): 395-397
ISSN: 0301-1208
Source:IJBB Vol.42(6) [December 2005]

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