Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/3532
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dc.contributor.authorDubey, Ritesh Kumar-
dc.contributor.authorTripathi, D N-
dc.date.accessioned2009-03-30T08:45:06Z-
dc.date.available2009-03-30T08:45:06Z-
dc.date.issued2005-10-
dc.identifier.issn0301-1208-
dc.identifier.urihttp://hdl.handle.net/123456789/3532-
dc.description301-307en_US
dc.description.abstractThe thermal denaturation/renaturation processes in E. coli and eukaryotic DNAs have been studied using the laser light scattering (LS) technique. The differential scattering intensity curve has been utilized to determine the transition temperature Tm. The effect of solution pH on DNA thermal denaturation has been examined. It has been shown clearly that LS is an extremely sensitive method (more than the UV absorption method) and reveals even the subtler effects such as the pre-transition fluctuations, and that the DNA denaturation is prominently affected by pH. The dependence of melting temperature (Tm) on composition of DNA, number of base pairs and base sequences has also been investigated. It has been observed that depending upon its base sequence, the Tm¬ decreases in the case of renatured DNAs. The results have been compared with the UV absorption studies.en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.relation.ispartofseriesC07H21/04en_US
dc.sourceIJBB Vol. 42(5) [October 2005]en_US
dc.subjectLaser light scatteringen_US
dc.subjectDNAen_US
dc.subjectDenaturationen_US
dc.subjectRenaturationen_US
dc.subjectE. colien_US
dc.subjectEukaryotic DNAen_US
dc.titleA study of thermal denaturation/renaturation in DNA using laser light scattering: A new approachen_US
dc.typeArticleen_US
Appears in Collections:IJBB Vol.42(5) [October 2005]

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