NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.42 [2005] >
IJBB Vol.42(5) [October 2005] >


Title: A study of thermal denaturation/renaturation in DNA using laser light scattering: A new approach
Authors: Dubey, Ritesh Kumar
Tripathi, D N
Keywords: Laser light scattering
DNA
Denaturation
Renaturation
E. coli
Eukaryotic DNA
Issue Date: Oct-2005
Publisher: CSIR
IPC CodeC07H21/04
Abstract: The thermal denaturation/renaturation processes in E. coli and eukaryotic DNAs have been studied using the laser light scattering (LS) technique. The differential scattering intensity curve has been utilized to determine the transition temperature Tm. The effect of solution pH on DNA thermal denaturation has been examined. It has been shown clearly that LS is an extremely sensitive method (more than the UV absorption method) and reveals even the subtler effects such as the pre-transition fluctuations, and that the DNA denaturation is prominently affected by pH. The dependence of melting temperature (Tm) on composition of DNA, number of base pairs and base sequences has also been investigated. It has been observed that depending upon its base sequence, the TmĀ¬ decreases in the case of renatured DNAs. The results have been compared with the UV absorption studies.
Page(s): 301-307
ISSN: 0301-1208
Source:IJBB Vol.42(5) [October 2005]

Files in This Item:

File Description SizeFormat
IJBB 42(5) 301-307.pdf160.19 kBAdobe PDFView/Open
 Current Page Visits: 630 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 559268 since 06-Feb-2009  Last updated on 30-Jul-2014Webmaster: nopr@niscair.res.in