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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.42 [2005] >
IJBB Vol.42(4) [August 2005] >
| Title: | Occurrence of trypsin-like protease in cardamom (Elettaria cardamomum Maton) |
| Authors: | Josephrajkumar, A
Chakrabarty, Romit
Thomas, George |
| Keywords: | Cardamom
Elettaria cardamomum Maton
Trypsin
Aprotinin. |
| Issue Date: | Aug-2005 |
| Publisher: | CSIR |
| IPC Code: | A61K38/43, C12N9/00 |
| Abstract: | Occurrence of trypsin-like protease in fresh cardamom (Elettaria cardamomum Maton) seeds, as evidenced by the benzoyl-arg-p-nitroanilide (BApNA) hydrolyzing ability of the seed enzyme preparation under alkaline condition is reported for the first time. The enzyme has a pH and temperature optima as 8 and 45ºC, respectively. It is inhibited by aprotinin and phenylmethyl sulfonyl fluoride (PMSF) in a dose-dependent manner, suggesting the presence of serine residues at the active site. The enzyme had a Vmax of 98.01 nmoles p-nitroaniline released per min per mg protein and Km of 0.0684 mM with BApNA as substrate. Addition of aprotinin (75.75 μM) increased Km value by three-folds, whereas the Vmax was reduced by 23%. |
| Page(s): | 243-245 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.42(4) [August 2005]
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