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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.42 [2005] >
IJBB Vol.42(4) [August 2005] >
| Title: | Effect of phenobarbitone on cytochrome P450 activity and chlorpyrifos and 3,5,6-trichloropyridinol levels in liver and serum in rat |
| Authors: | Verma, Radhey S
Mehta, Anugya
Srivastava, N |
| Keywords: | Organophosphate pesticides
Chlorpyrifos
Cytochrome P450
3,5,6-trichloropyridinol
Pheno-barbitone
Hepatic microsome
Cytochrome P450 induction
Serum
Liver
Rat |
| Issue Date: | Aug-2005 |
| Publisher: | CSIR |
| IPC Code: | A01N55/00 |
| Abstract: | Chlorpyrifos [O,O'-diethyl-O-(3,5,6-trichloro-2-pyridyl) phos-phorothionate, CPF] undergoes oxidative desulfuration or dearylation by hepatic microsomal cytochrome P450 (CYP)-mediated monooxygenase reaction to CPF oxon or desethyl CPF, which are further metabolized to 3,5,6-trichloropyridinol (TCP). The present study showed that CPF exposure caused induction of hepatic CYP levels in rats. Phenobarbitone (PB) treatment elevated CYP activity by about 2.2-folds in controls, while CPF exposure to PB-treated rats did not cause further elevation in CYP levels. The levels of CPF in liver tissue and serum of rats given 50 and 100 mg CPF/kg body wt for 3 weeks were 17.15 ng and 29.39 ng/g and 33.71 ng and 56.34 ng/ml, respectively. The levels of TCP in these rats were 123.58 ng and 215.26 ng/g in liver tissue and 391.73 ng and 599.32 ng/ml in serum respectively. On PB-treatment, CPF levels were decreased by 46% and 38% in liver and 23% and 20% in serum of rats receiving 50 mg and 100 mg CPF/kg body wt for 3 days, while TCP levels were increased by 6% and 22% in liver and 22% and 44% serum, respectively. Results of this study clearly show that CYP2B, the PB-inducible form can biotransform CPF faster into TCP. |
| Page(s): | 254-257 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.42(4) [August 2005]
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