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Title: Characterization and purification of alkaline phosphatase from Elephas trogontherii (Steppe elephant) bone
Authors: Demir, Yaşar
Yildirim, Safinur
Demir, Nazan
Keywords: Elephas trogontherii
Alkaline phos-phatase
Issue Date: Jun-2005
Publisher: CSIR
IPC CodeA 01 N 63/00, C 12 N
Abstract: Four isozymes of alkaline phosphatase (AP) were purified from Elephas trogontherii (Steppe elephant) from different locations in the bone (outer and inner peripheral, cytosolic, and integral) using Sephadex G-200 gel filtration and TEAE-cellulose anion-exchange chromatography. The specimen was obtained from Erzurum Museum and its age was approx. 0.3-0.5 million years old. No fungi or bacteria were present in the bone sample. The enzyme activity was determined by using p-nitrophenylphosphate as a substrate. SDS-PAGE of all the isozymes gave a single band at the same location. The molecular mass of the four isozymes as determined by using gel filtration was about 60 kDa. Optimum pHs for the four isozymes were between 8-8.5. The optimum temperatures of the isozymes were: outer peripheral, 37.5ºC, cytosolic, 37.5ºC, inner peripheral, 35ºC and integral, 40ºC. The values of Vmax and Km, as well different optimum temperatures indicated that isozymes were structurally different.
Page(s): 182-185
ISSN: 0301-1208
Source:IJBB Vol.42(3) [June 2005]

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