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dc.contributor.authorElsayed, E A-
dc.contributor.authorOmar, H G-
dc.contributor.authorGalil, S A-
dc.contributor.authorEl-Enshasy, H A-
dc.identifier.issn0975-1084 (Online); 0022-4456 (Print)-
dc.description.abstractAlpha amylase (E.C. is one of the most important starch-degrading enzymes and widely applied in pharmaceutical, food, feed, detergent, textile and bio refinery industries. The present work was focused on optimization of ɑ-amylase production using the bacteria strain Bacillus amyloliquefaciens in submerged cultivation system. The results showed that, the optimal soluble starch concentration for maximal enzyme production of 1950 µkat.L-1 was 20 g.L-1. Compared to shake flask, bioreactor batch cultivation yielded higher volumetric enzyme production of 4125 µkat.L-1 (about 1.84-folds higher than the value obtained in shake flask). In addition, fed-batch cultivation with two feeding strategies (constant feeding and increased feeding) was also designed to improve the production process. The maximal volumetric enzyme production of 8160 µkat.L-1 was obtained in increased feeding strategy after 36 hours of cultivation. This value was almost 98 and 18% higher than the enzyme produced in batch and constant feeding fed-batch cultivations, respectively.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rightsCC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceJSIR Vol.75(08) [August 2016]en_US
dc.subjectBacillus amyloliquefaciensen_US
dc.subjectSubmerged cultureen_US
dc.subjectBatch cultivationen_US
dc.subjectFed-batch cultivationen_US
dc.titleOptimization of fed-batch cultivation strategy for extracellular ɑ-amylase production by Bacillus amyloliquefaciens in submerged cultureen_US
Appears in Collections:JSIR Vol.75(08) [August 2016]

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