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Title: Optimization of fed-batch cultivation strategy for extracellular ɑ-amylase production by Bacillus amyloliquefaciens in submerged culture
Authors: Elsayed, E A
Omar, H G
Galil, S A
El-Enshasy, H A
Keywords: Bacillus amyloliquefaciens;Submerged culture;ɑ-amylase;Bioreactor;Batch cultivation;Fed-batch cultivation
Issue Date: Aug-2016
Publisher: NISCAIR-CSIR, India
Abstract: Alpha amylase (E.C. is one of the most important starch-degrading enzymes and widely applied in pharmaceutical, food, feed, detergent, textile and bio refinery industries. The present work was focused on optimization of ɑ-amylase production using the bacteria strain Bacillus amyloliquefaciens in submerged cultivation system. The results showed that, the optimal soluble starch concentration for maximal enzyme production of 1950 µkat.L-1 was 20 g.L-1. Compared to shake flask, bioreactor batch cultivation yielded higher volumetric enzyme production of 4125 µkat.L-1 (about 1.84-folds higher than the value obtained in shake flask). In addition, fed-batch cultivation with two feeding strategies (constant feeding and increased feeding) was also designed to improve the production process. The maximal volumetric enzyme production of 8160 µkat.L-1 was obtained in increased feeding strategy after 36 hours of cultivation. This value was almost 98 and 18% higher than the enzyme produced in batch and constant feeding fed-batch cultivations, respectively.
Page(s): 480-486
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.75(08) [August 2016]

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