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Title: | Development of transgenic pigeonpea (Cajanus cajan. L Millsp) overexpressing citrate synthase gene for high phosphorus uptake |
Authors: | Hussain, Aftab IS Pavithra, HV Sreevathsa, Rohini Nataraja, KN Babu, Naveen |
Keywords: | Crop productivity;Soil nutrients |
Issue Date: | Aug-2016 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | Plants have developed several adaptive strategies to enhance the availability and uptake of phosphorus (P) from the soil under conditions of P deficiency. Exudation of organic acids like citrate is one of the important strategies. In this study, we developed transgenic pigeonpea (Cajanus cajan) over-expressing Dacus carota citrate synthase (DcCs) gene to increase the synthesis and exudation of citrate. Transgenic plants were generated through agro bacterium mediated in-planta transformation technique. Integration and expression of the transgene was confirmed by genomic Southern and RT-PCR analysis. We observed that the transgenic lines had more tissue P and chlorophyll content, and also citrate synthase content higher in the roots. Further, transgenic lines had more vigorous root system both under P sufficient and deficient conditions with more lateral roots and root hairs under P deficient conditions. We conclude that the transgenic pigeonpea plants have the capacity to acquire more P under P deficient conditions. |
Page(s): | 493-501 |
URI: | http://nopr.niscair.res.in/handle/123456789/35140 |
ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
Appears in Collections: | IJEB Vol.54(08) [August 2016] |
Files in This Item:
File | Description | Size | Format | |
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IJEB 54(8) 493-501.pdf | 203.28 kB | Adobe PDF | View/Open |
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