Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/35135
Title: Isolation, phylogenetic diversity and screening of bioprospecting potentials of ascidians - associated bacteria from Gulf of Mannar, Southeast coast of India
Authors: Ananthan, Gnanakkan
Sathishkumar, Ramamoorthy
Keywords: Ascidians associated bacteria;Extracellular enzymes;Antimicrobials;Phylogeny;Southeast coast of India
Issue Date: Jul-2016
Publisher: NISCAIR-CSIR, India
Abstract: A total of 93 culturable heterotrophic bacteria were isolated from eight ascidians species. Among these, 10 isolates showed extracellular enzymatic and antimicrobial activities against pathogenic bacteria. These ascidian associated potential bacteria were further investigated on their phenotype characters and 16S rRNA genes sequence analysis. Ascidians-associated bacterial strain could be subdivided into two phylogenetically different clusters; two strains were affiliated with Firmicutes (genera Bacillus and Marinobacter sp.) and eight with a-Proteobacteria (Vibrio sp.). Potential activities of the three enzymes (protease, lipase and amylase) were recorded from B. subtilis GA CAS8 and Marinobacter sp. GA CAS9. Ascidian-associated bacterial strains, B. subtilis GA CAS8 and Bacillus sp. GA CAS1 exhibited higher antimicrobial activity against both Gram-negative, Gram-positive bacteria and fungi including B. subtilis, B. cereus, S. aureus, V. harveyi, P. aeruginosa and Candida albicans.
Page(s): 889-896
URI: http://nopr.niscair.res.in/handle/123456789/35135
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.45(07) [July 2016]

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