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Title: Assessment of genetic fidelity in in vitro regenerated plants of Brassica juncea (L.) Czern & Coss. using RAPD and ISSR markers
Authors: Verma, V
Thakur, A K
Singh, B K
Chauhan, D K
Singh, K H
Chauhan, J S
Keywords: Brassica juncea;Clonal fidelity;Cotyledon explants;Molecular markers;Regeneration;Somaclonal variation
Issue Date: Jan-2016
Publisher: NISCAIR-CSIR, India
Abstract: RAPD and ISSR markers were used to assess the genetic integrity of Indian mustard var. NRCDR-2 plants regenerated from cotyledon explants via direct organogenesis. Of 60 (40 RAPD & 20 ISSR) markers screened, only 27 RAPD and 12 ISSR markers produced a total of 286 (198 RAPD and 88 ISSR) clear, distinct and reproducible amplicons. All the RAPD markers produced a total of 198 bands, ranging 50 to 8000 bp length and the number of scorable bands varied 3 to 15 per primer with an average of 7.33 bands per primer. Whereas, all the ISSRs produced a total of 88 amplicons in the size range of 300 to 3000 bp and 4 to 12 scorable bands per primer with an average of 7.33 bands per primer. Analysis of RAPD and ISSR banding patterns generated by PCR amplification gave zero evidence for the presence of somaclonal variations. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. This homogenous amplification-profile confirmed the clonal fidelity of the tissue culture raised Indian mustard plants.
Page(s): 120-123
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.15(1) [January 2016]

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