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Title: In vitro micropropagation of Simarouba glauca DC.
Authors: Lavanya, A R
Muthukumar, M
Muthukrishnan, S
Kumaresan, V
Kumar, T Senthil
Rao, A S
Rao, M V
Keywords: Additives;Growth regulators;Micropropagation;Simarouba glauca;Shoot multiplication
Issue Date: Jan-2016
Publisher: NISCAIR-CSIR, India
Abstract: An efficient and improved micropropagation protocol was developed from different explants of 2-month-old seedlings of Simarouba glauca DC. The highest number of shoots (55.3) with 100% response was obtained from cotyledonary-node explant on MS medium fortified with 6-benzyl adenine (BA; 3.0 mg/L), kinetin (KN; 0.5 mg/L), α-naphthalene acetic acid (NAA; 0.5 mg/L) and glutamine (10 mg/L). Further, the highest rooting response (95%) with 15 roots of 5.8 cm root length was observed on MS medium supplemented with NAA (3.0 mg/L). The rooted plantlets were hardened by transferring to paper containers with soil and sand (2:1) and kept in shade for acclimatization. These plants were further transferred to the field with a survival percentage of 81.5%.
Page(s): 107-111
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.15(1) [January 2016]

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