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dc.contributor.authorSanthosh, Ramachandran Sarojini-
dc.contributor.authorDharmalingam, Kuppamuthu-
dc.identifier.issn0975-0967 (Online); 0972-5849 (Print)-
dc.description.abstractMammalian cell entry gene of mycobacterium helps its entry into epithelial and natural target cells. In order to carry out the functional assay of Mycobacterium leprae Mce1A, the whole gene was cloned in Escherichia coli. An overexpression vector carrying M. leprae mce1A gene, under IPTG inducible T5 promoter, was cloned in E. coli for expression of encoded protein with N-terminal 6xHis-tag. In the absence of IPTG, E. coli cells carrying the mce1A gene grew normally but induction of gene expression led to inhibition of cell growth. Western blot analysis using anti-His HRP conjugate showed full length Mce1A protein expressed in low amount. Deletion of N-terminal region having adjacent arginine rare codons resulted in overexpression of truncated protein as inclusion bodies without inhibiting cell division with size reduction of recombinant protein. However the full length protein poorly expressed without size reduction.en_US
dc.publisherNISCAIR-CSIR, India-
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBT Vol.15(1) [January 2016]en_US
dc.subjectCodon usageen_US
dc.subjectMce1A proteinen_US
dc.subjectMycobacterium lepraeen_US
dc.subjectRare codonsen_US
dc.titleRare codons caused poor expression of mammalian cell entry (Mce1A) gene cloned from Mycobacterium leprae in Escherichia colien_US
Appears in Collections:IJBT Vol.15(1) [January 2016]

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