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Title: Genetic diversity and variation of Vibrio from Korea by pulsed-field gel electrophoresis
Authors: Huh, Man Khu
Keywords: Vibrio cholera;V. parahaemolyticus;V. vulnificus;Fish;Mollusks
Issue Date: Sep-2014
Publisher: NISCAIR-CSIR, India
Abstract: Samples contaminated with Vibrio cholera, V. parahaemolyticus and V. vulnificus were identified using PCR with primers.  Three species was found in the examined fish and mollusk at all markets in Busan and was found in all seasons. In most cases, the bacteria were isolated from the epithelial intestines of fish. Only three species of mollusks were contaminated with V. parahaemolyticus. V. vulnificus was found in Sebastes pachycephalus (Jacopever, Schlegel's blac rockfish) and Anguilla japonica (Japanese eel). For optimization of electrophoretic parameters of PFGE, isolates of 6 sites were analyzed with NotI digestion. NotI could be used as a restriction enzyme in PFGE subtyping of L. pneumophila.
Page(s): 1682-1688
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.43(09) [September 2014]

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