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dc.contributor.authorChennappa, G-
dc.contributor.authorNaik, MK-
dc.contributor.authorAdkar-Purushothama, CR-
dc.contributor.authorAmaresh, YS-
dc.contributor.authorSreenivasa, MY-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractAzotobacter strains were isolated by serial dilution method and colonies were viscous, smooth, glistening, and brown to black colour on Jenson’s N-free agar. Morphological and biochemical tests showed characteristic features of Azotobacter. Further, molecular analyses revealed the presence of different Azotobacter species viz., A. armeniacus, A. chroococcum, A. salinestris, A. tropicalis and A. vinelandii. The isolates were tested for their ability of nitrogen fixation, indole acetic acid (IAA), gibberllic acid production and phosphate solubilization. Four isolates (GVT-1, GVT-2 KOP-11 and SND-4) were efficient in fixation of highest amount of N2 (29.21 μg NmL-1day-1), produced IAA (25.50 µg mL-1), gibberllic acid (17.25 μg 25 mL-1) and formed larger P solubilizing zone (13.4 mm). Some of the Azotobacter strains were produced siderophores, hydrogen cyanide and were positive for ammonia production with respect to antifungal activity of Azotobacter was tested with dual culture method and A. tropicalis inhibited the growth of Fusarium, Aspergillus and Alternaria species. Azotobacter isolates were tested against salt (0-10%), temperature (4-55ºC), pH (5.0-10) and insecticide chloropyrifos (0-3%) tolerance study. Among them, A. chroococcum was found tolerant to a maximum of 6% NaCl with a temperature of 35-45ºC and to a pH up to 8. All the 4 strains showed effective growth against 3% chloropyrifos concentration. The studies revealed that the Azotobacter strains not only produced plant growth promoting substances but are also tolerant to abiotic stresses such as temperature, pH and insecticides.en_US
dc.publisherNISCAIR-CSIR, India-
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.54(05) [May 2016]en_US
dc.subjectGibberllic aciden_US
dc.subjectIndole acetic acid (IAA)en_US
dc.subjectNitrogen fixationen_US
dc.titlePGP potential, abiotic stress tolerance and antifungal activity of Azotobacter strains isolated from paddy soilsen_US
Appears in Collections:IJEB Vol.54(05) [May 2016]

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