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Title: In vitro seed germination and plant regeneration of an epiphytic orchid Aerides ringens (Lindl.) Fischer
Authors: Srivastava, Deepti
Gayatri, M C
Sarangi, S K
Keywords: Aerides ringens;6-benzyl amino purine;Knudson C;Murashige and Skoog basal medium;Peptone;Protocorms
Issue Date: Oct-2015
Publisher: NISCAIR-CSIR, India
Abstract: Aerides ringens (Lindl.) Fischer is an epiphytic and endemic orchid of Western Ghats, India, which is popular for its beautiful flowers and fox-tail inflorescence. The present study offers novel and reproducible protocol for in vitro seed germination of A. ringens. Seed viability was determined by triphenyl tetrazolium chloride and fluorescein diacetate staining methods and 88.86%±6.7 seeds were found viable at the time of inoculation. Viable seeds were then evaluated for germination on Murashige and Skoog (MS) basal medium and Knudson C (KC) medium supplemented with variable concentrations of 6-benzyl amino purine (BAP) and peptone. Seed culture on KC medium supplemented with 4.44 μM BAP and 500 mg L-1 peptone exhibited best rate of seed germination (89.28±3.42%), which transformed into green colour vigorous and compact protocorms of 1.89±0.38 mm size. Protocorms were subcultured on KC medium fortified with different concentrations and combination of cytokinins [BAP, kinetin (KN) and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron; TDZ) and auxin (IAA)]. Best results were obtained in the presence of Kn (9.3 μM), which produced 4.40±2.20 shoots per segment of 3.05±0.46 cm length. Seedlings were transferred to KC medium fortified with auxins (IAA, NAA & IBA) for rooting. Half strength KC with IAA (5.71 μM) produced strong and stout 4.44±1.61 roots of 3.22±0.40 cm length per plantlet. Harden and acclimatized plants were established ex vitro with 80% survival rate.
Page(s): 574-580
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(4) [October 2015]

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