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Title: Effect of explant origin on clonal propagation of Aegle marmelos (L.) Corr. —A multipurpose tree species
Authors: Parveen, Shahina
Shahzad, Anwar
Nazim, Farah
Keywords: Bael;Chlorophyll;Micropropagation;Nodal explant;Rooting;Root sucker explant
Issue Date: Oct-2015
Publisher: NISCAIR-CSIR, India
Abstract: A comparative analysis was performed on the clonal propagation from aerial nodal segments and root sucker derived nodal segments of Aegle marmelos (L.) Corr. on MS medium supplemented with various concentrations of plant growth regulators (PGRs). The results proved that the nodal segments from root suckers have great potentials to multiply rapidly and produced large number of shoots (12.4±0.7) compared to aerial nodal segments (5.8±0.6) on a similar medium (MS+BA 5.0 µM+NAA 0.5 µM) and culture conditions (6 wk). Cultures were maintained up to the 5th subculture stage on the optimal regeneration medium for further multiplication and proliferation and by the end of 3rd subculture passage, a maximum of 40.4±3.6 shoots/explant were achieved from root sucker derived nodal segments. Ex vitro rooting in microshoots was employed to shorten the propagation span, with an added effect on acclimatization success. During acclimatization, estimation of different photosynthetic pigments was also carried out.
Page(s): 566-573
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(4) [October 2015]

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