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Title: Cyclic somatic embryogenesis of elite Indian cassava variety H-226
Authors: Anuradha, T
Kumar, K K
Balasubramanian, P
Keywords: Cassava mosaic virus;Cyclic somatic embryogenesis;Indian cassava var. H-226
Issue Date: Oct-2015
Publisher: NISCAIR-CSIR, India
Abstract: A method to induce high frequency somatic embryogenesis was investigated in elite Indian cassava variety H-226. Highest (66.77%) primary somatic embryogenesis was obtained from immature leaf lobes with Picloram 12 mg L-1 with slight modification of MS medium components with B5 vitamins and 3% maltose (SEIM; somatic embryo induction medium). The same medium provided the highest (80%) secondary embryogenesis using somatic cotyledons as explants. Thus, a two-step medium sequence was developed for efficient regeneration of somatic embryos. Primary and somatic embryos matured in somatic embryo maturation medium (SEMM; MS basal medium with 0.2 mg L-1 BAP and 0.01 mg L-1 NAA) developed normal shoot and roots in regeneration medium (RGM; MS basal medium with 0.2 mg L-1 BAP, 0.01 mg L-1 NAA and 0.2 mg L-1 GA3). The maturation and regeneration frequencies were 80 and 85.25%, respectively. Through secondary (cyclic) somatic embryogenesis protocol, the in vitro cassava plants could be obtained in a period of 6 months and it shortened the period of regeneration in cassava right from explant to plantlet development by 4 months. The regenerated cassava plantlets were morphologically normal and acclimatized in the field with a survival rate of 80%. The present regeneration protocol has immense potential in developing transgenic cassava resistant to deadly cassava mosaic virus (CMV) and for improvement of other economically important traits in cassava.
Page(s): 559-565
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(4) [October 2015]

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